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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
11/02/2015 |
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Data da última atualização: |
22/02/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MIDORIKAWA, G. E. O.; SOUZA, M. de L. M. de; SILVA, O. F.; DIAS, J. do S. A.; KANZAKI, L. I. B.; HANADA, R. E.; MESQUITA, R. M. L. C.; GONCALVES, R. C.; ALVARES, V. S.; BITTENCOURT, D. M. C.; MILLER, R. N. G. |
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Afiliação: |
GLAUCIA E. O. MIDORIKAWA, UnB; MARIA DE LOURDES MENDES DE SOUZA, CTAA; OTNIEL FREITAS SILVA, CTAA; JUREMA DO SOCORRO AZEVEDO DIAS, CPAF-AP; LUIS I. B. KANZAKI, UnB; ROGERIO E. HANADA, INPA; RENATA M. L. C. MESQUITA, INPA; RIVADALVE COELHO GONCALVES, CPAF-AC; VIRGINIA DE SOUZA ALVARES, CPAF-AC; DANIELA MATIAS DE C BITTENCOURT, CPAA; ROBERTO N. G. MILLER, UnB. |
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Título: |
Characterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Microbiology, v. 14, n. 138, 2014. |
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DOI: |
10.1186/1471-2180-14-138 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius and A. flavus observed on Brazil nut, and associated risk of mycotoxin accumulation, simple identification methods for such mycotoxigenic species are of importance for Hazard Analysis Critical Control Point system implementation. The assay for the genus Aspergillus represents progress towards specific PCR identification and detection of mycotoxigenic species. MenosBackground: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius ... Mostrar Tudo |
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Palavras-Chave: |
Castanha-do-brasil; Pragas e doenças. |
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Thesagro: |
Aflatoxina. |
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Thesaurus Nal: |
Aspergillus. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/102994/1/castanha-Robert.pdf
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Marc: |
LEADER 02820naa a2200301 a 4500
001 2008479
005 2016-02-22
008 2014 bl --- 0-- u #d
024 7 $a10.1186/1471-2180-14-138$2DOI
100 1 $aMIDORIKAWA, G. E. O.
245 $aCharacterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level.$h[electronic resource]
260 $c2014
520 $aBackground: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius and A. flavus observed on Brazil nut, and associated risk of mycotoxin accumulation, simple identification methods for such mycotoxigenic species are of importance for Hazard Analysis Critical Control Point system implementation. The assay for the genus Aspergillus represents progress towards specific PCR identification and detection of mycotoxigenic species.
650 $aAspergillus
650 $aAflatoxina
653 $aCastanha-do-brasil
653 $aPragas e doenças
700 1 $aSOUZA, M. de L. M. de
700 1 $aSILVA, O. F.
700 1 $aDIAS, J. do S. A.
700 1 $aKANZAKI, L. I. B.
700 1 $aHANADA, R. E.
700 1 $aMESQUITA, R. M. L. C.
700 1 $aGONCALVES, R. C.
700 1 $aALVARES, V. S.
700 1 $aBITTENCOURT, D. M. C.
700 1 $aMILLER, R. N. G.
773 $tBMC Microbiology$gv. 14, n. 138, 2014.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
08/12/1999 |
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Data da última atualização: |
29/07/2015 |
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Autoria: |
RESENDE, F. V.; FAQUIN, V.; SOUZA, R. J. de; SANTOS, V. S. |
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Afiliação: |
FRANCISCO VILELA RESENDE, CNPH; V. FAQUIN.; R. J. de SOUZA; V. S. SANTOS. |
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Título: |
Acumulo de materia seca e exigencias nutricionais de plantas de alho provenientes de cultura de tecidos e de propagação convencional. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Horticultura Brasileira, Brasília, DF, v. 17, n. 3, p. 220-226, nov. 1999. |
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ISSN: |
0102-9991 |
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Idioma: |
Português |
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Palavras-Chave: |
Dry matter; In vitro multiplication; Mineral nutrition; Multiplicacao in vitro; Nutricao mineral. |
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Thesagro: |
Alho; Allium Sativum; Cultura de Tecido; Matéria Seca. |
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Thesaurus NAL: |
garlic; tissue culture. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/127122/1/19991738.pdf
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Marc: |
LEADER 00876naa a2200289 a 4500
001 1766151
005 2015-07-29
008 1999 bl uuuu u00u1 u #d
022 $a0102-9991
100 1 $aRESENDE, F. V.
245 $aAcumulo de materia seca e exigencias nutricionais de plantas de alho provenientes de cultura de tecidos e de propagação convencional.
260 $c1999
650 $agarlic
650 $atissue culture
650 $aAlho
650 $aAllium Sativum
650 $aCultura de Tecido
650 $aMatéria Seca
653 $aDry matter
653 $aIn vitro multiplication
653 $aMineral nutrition
653 $aMultiplicacao in vitro
653 $aNutricao mineral
700 1 $aFAQUIN, V.
700 1 $aSOUZA, R. J. de
700 1 $aSANTOS, V. S.
773 $tHorticultura Brasileira, Brasília, DF$gv. 17, n. 3, p. 220-226, nov. 1999.
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Embrapa Hortaliças (CNPH) |
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